Quickstart

Use this section to test the runability of the pipeline
A more comprehensive step-by-step-guide for the setup of the pipeline and the test on real data can be found in the Installation section.

Requirements

See Requirements. Make sure to have Nextflow and Singularity or Docker installed

Run quicksand

Test the runability of the quicksand pipeline using the included test-data:

nextflow run mpieva/quicksand -profile singularity,test
See the final_report.tsv for a summary of the results.
See the Output section for a detailed explaination of all the output files.

Troubleshooting

  • heap-space error: Nextflow related error, not enough memory allocated for the Java Virtual machine to run the processes. Manually set the memory for nextflow by typing export NXF_OPTS="-Xms128g -Xmx128g" before the run

  • file-not found error: Your Singularity installation might not allow auto-mounting of paths: See Singularity